A conditional knockout resource for the genome-wide study of mouse gene function WC Skarnes, B Rosen, AP West, M Koutsourakis, W Bushell, V Iyer, ... Nature 474 (7351), 337-342, 2011 | 1890 | 2011 |
Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting J Fu, X Bian, S Hu, H Wang, F Huang, PM Seibert, A Plaza, L Xia, ... Nature biotechnology 30 (5), 440-446, 2012 | 511 | 2012 |
Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice K Anastassiadis, J Fu, C Patsch, S Hu, S Weidlich, K Duerschke, ... Disease models & mechanisms 2 (9-10), 508-515, 2009 | 348 | 2009 |
Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1 K Daniel, J Lange, K Hached, J Fu, K Anastassiadis, I Roig, HJ Cooke, ... Nature cell biology 13 (5), 599-610, 2011 | 256 | 2011 |
Heterologous expression of a myxobacterial natural products assembly line in pseudomonads via red/ET recombineering SC Wenzel, F Gross, Y Zhang, J Fu, AF Stewart, R Müller Chemistry & biology 12 (3), 349-356, 2005 | 239 | 2005 |
The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation AS Bledau, K Schmidt, K Neumann, U Hill, G Ciotta, A Gupta, DC Torres, ... Development 141 (5), 1022-1035, 2014 | 205 | 2014 |
An improved recombineering approach by adding RecA to λ red recombination J Wang, M Sarov, J Rientjes, J Hu, H Hollak, H Kranz, Y Xie, AF Stewart, ... Molecular biotechnology 32, 43-53, 2006 | 198 | 2006 |
Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition J Fu, SC Wenzel, O Perlova, J Wang, F Gross, Z Tang, Y Yin, AF Stewart, ... Nucleic Acids Research 36 (17), e113-e113, 2008 | 178 | 2008 |
An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase A Kranz, J Fu, K Duerschke, S Weidlich, R Naumann, AF Stewart, ... genesis 48 (8), 512-520, 2010 | 169 | 2010 |
Transposon-mediated BAC transgenesis in human ES cells M Rostovskaya, J Fu, M Obst, I Baer, S Weidlich, H Wang, AJH Smith, ... Nucleic acids research 40 (19), e150-e150, 2012 | 163 | 2012 |
Meiotic DNA double-strand breaks and chromosome asynapsis in mice are monitored by distinct HORMAD2-independent and-dependent mechanisms L Wojtasz, JM Cloutier, M Baumann, K Daniel, J Varga, J Fu, ... Genes & development 26 (9), 958-973, 2012 | 163 | 2012 |
Improved seamless mutagenesis by recombineering using ccdB for counterselection H Wang, X Bian, L Xia, X Ding, R Müller, Y Zhang, J Fu, AF Stewart Nucleic acids research 42 (5), e37-e37, 2014 | 151 | 2014 |
Single-stranded heteroduplex intermediates in λ Red homologous recombination M Maresca, A Erler, J Fu, A Friedrich, Y Zhang, A Stewart BMC molecular biology 11, 1-15, 2010 | 150 | 2010 |
The histone demethylase UTX regulates stem cell migration and hematopoiesis S Thieme, T Gyárfás, C Richter, G Özhan, J Fu, D Alexopoulou, ... Blood, The Journal of the American Society of Hematology 121 (13), 2462-2473, 2013 | 130 | 2013 |
In vivo evidence for a prodrug activation mechanism during colibactin maturation X Bian, J Fu, A Plaza, J Herrmann, D Pistorius, AF Stewart, Y Zhang, ... ChemBioChem 14 (10), 1194-7, 2013 | 124 | 2013 |
Reconstitution of the Myxothiazol Biosynthetic Gene Cluster by Red/ET Recombination and Heterologous Expression in Myxococcus xanthus O Perlova, J Fu, S Kuhlmann, D Krug, AF Stewart, Y Zhang, R Müller Applied and Environmental Microbiology 72 (12), 7485-7494, 2006 | 111 | 2006 |
Biallelic expression of nanog protein in mouse embryonic stem cells A Filipczyk, K Gkatzis, J Fu, PS Hoppe, H Lickert, K Anastassiadis, ... Cell stem cell 13 (1), 12-13, 2013 | 106 | 2013 |
A recombineering pipeline to make conditional targeting constructs J Fu, M Teucher, K Anastassiadis, W Skarnes, AF Stewart Methods in enzymology 477, 125-144, 2010 | 101 | 2010 |
Metabolic engineering of Pseudomonas putida for methylmalonyl-CoA biosynthesis to enable complex heterologous secondary metabolite formation F Gross, MW Ring, O Perlova, J Fu, S Schneider, K Gerth, S Kuhlmann, ... Chemistry & biology 13 (12), 1253-1264, 2006 | 82 | 2006 |
High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes AW Bird, A Erler, J Fu, JK Hériché, M Maresca, Y Zhang, AA Hyman, ... Nature methods 9 (1), 103-109, 2012 | 78 | 2012 |