Michael W. Pfaffl

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Jo Vandesompeleprofessor in functional cancer genomics and applied bioinformaticsVerifisert e-postadresse på ugent.be
Mikael KubistaInstitute of Biotechnology, CAS, Life Genomics ABVerifisert e-postadresse på ibt.cas.cz
Benedikt KirchnerPost-Doc TU MünchenVerifisert e-postadresse på tum.de
Stephen BustinProfessor of Molecular Medicine, Anglia Ruskin UniversityVerifisert e-postadresse på aru.ac.uk
Vladimir BenesEMBLVerifisert e-postadresse på embl.de
Jim F HuggettNational Measurement Laboratory/University of SurreyVerifisert e-postadresse på lgcgroup.com
Jeremy GarsonUCLVerifisert e-postadresse på ucl.ac.uk
Carl WittwerProfessor of Pathology, University of UtahVerifisert e-postadresse på path.utah.edu
Dominik BuschmannPostdoctoral Fellow at Johns Hopkins MedicineVerifisert e-postadresse på wzw.tum.de
Bajram BerishaUniversity of PrishtinaVerifisert e-postadresse på uni-pr.edu
Veronika MussackVerifisert e-postadresse på wzw.tum.de
Fred SinowatzProfessor of Anatomy, Histology and Embryology, University of MunichVerifisert e-postadresse på anat.vetmed.uni-muenchen.de
Susanne UlbrichVerifisert e-postadresse på usys.ethz.ch
Agnes S. MeidertLMU MunichVerifisert e-postadresse på med.uni-muenchen.de
Matthias KleinLudwig Maximilians UniversityVerifisert e-postadresse på med.uni-muenchen.de
Ivan Penchev GeorgievProfesor in Physiology, Trakia University, Stara Zagora, BulgariaVerifisert e-postadresse på uni-sz.bg
Sabine BartelERS/EU RESPIRE3 Marie Sklodowska-Curie FellowVerifisert e-postadresse på umcg.nl
Sebastian KaiserDepartment of Statistics, LMU MunichVerifisert e-postadresse på stat.uni-muenchen.de
Susanne Krauss-EtschmannResearch Center Borstel, Leibniz-Center for Medicine and BiosciencesVerifisert e-postadresse på fz-borstel.de
Andrej-Nikolai Spiess, PhDIndependent Researcher, FreelanceVerifisert e-postadresse på data-squeezer.de

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Michael W. Pfaffl

Professor of Molecular Physiology, TUM Technische Universität München Technical University of Munich

Verifisert e-postadresse på gene-quantification.info - Startside

Molecular Physiology qPCR Molecular Diagnostics Liquid Biopsy Transcriptomics


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A new mathematical model for relative quantification in real-time RT–PCR MW Pfaffl Nucleic Acids Research 29 (9), e45-e45, 2001	38029	2001
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ... Clinical Chemistry 55 (4), 611-622, 2009	16621	2009
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the … C Théry, KW Witwer, E Aikawa, MJ Alcaraz, JD Anderson, ... Journal of Extracellular Vesicles 7 (1), 1535750, 2018	9343	2018
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR MW Pfaffl, GW Horgan, L Dempfle Nucleic Acids Research 30 (9), e36-e36, 2002	8683	2002
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations MW Pfaffl, A Tichopad, C Prgomet, TP Neuvians Biotechnology Letters 26 (6), 509-515, 2004	5504	2004
RNA integrity and the effect on the real-time qRT-PCR performance S Fleige, MW Pfaffl Molecular Aspects of Medicine 27 (2-3), 126-139, 2006	1955	2006
Quantitative real-time RT-PCR – a perspective. SA Bustin, V Benes, T Nolan, MW Pfaffl Journal of Molecular Endocrinology 34 (3), 597-601, 2005	1939	2005
Quantification strategies in real-time PCR MW Pfaffl A-Z of Quantitative PCR edited by SA Bustin, 89-113, 2004	1460*	2004
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research J Van Deun, P Mestdagh, P Agostinis, Ö Akay, S Anand, J Anckaert, ... Nature Methods 14 (3), 228-232, 2017	1039	2017
The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ... Clinical Chemistry 59 (6), 892-902, 2013	917	2013
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper B Mateescu, EJK Kowal, BWM van Balkom, S Bartel, SN Bhattacharyya, ... Journal of Extracellular Vesicles 6 (1), 1286095, 2017	753	2017
Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR S Fleige, V Walf, S Huch, C Prgomet, J Sehm, MW Pfaffl Biotechnology Letters 28 (19), 1601-1613, 2006	743	2006
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches JA Welsh, DCI Goberdhan, L O'Driscoll, EI Buzas, C Blenkiron, ... Journal of extracellular vesicles 13 (2), e12404, 2024	703	2024
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments D Svec, A Tichopad, V Novosadova, MW Pfaffl, M Kubista Biomolecular Detection and Quantification 3, 9-16, 2015	677	2015
Standardized determination of real‐time PCR efficiency from a single reaction set‐up A Tichopad, M Dilger, G Schwarz, MW Pfaffl Nucleic Acids Research 31 (20), e122-e122, 2003	659	2003
Relative Quantification MW Pfaffl „Real-time PCR“ edited by M. Tevfik Dorak, 63-82, 2006	511	2006
Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR MW Pfaffl, M Hageleit Biotechnology letters 23, 275-282, 2001	411	2001
mRNA and microRNA quality control for RT-qPCR analysis C Becker, A Hammerle-Fickinger, I Riedmaier, MW Pfaffl Methods 50 (4), 237-243, 2010	361	2010
RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization T Hruz, M Wyss, M Docquier, MW Pfaffl, S Masanetz, L Borghi, ... BMC Genomics 12 (1), 156, 2011	347	2011
The need for transparency and good practices in the qPCR literature SA Bustin, V Benes, J Garson, J Hellemans, J Huggett, M Kubista, ... Nature Methods 10 (11), 1063-1067, 2013	317	2013

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