Aqueous two-phase systems: a viable platform in the manufacturing of biopharmaceuticals PAJ Rosa, IF Ferreira, AM Azevedo, MR Aires-Barros Journal of Chromatography A 1217 (16), 2296-2305, 2010 | 314 | 2010 |
Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing AM Azevedo, PAJ Rosa, IF Ferreira, MR Aires-Barros Trends in biotechnology 27 (4), 240-247, 2009 | 310 | 2009 |
Cutinase: from molecular level to bioprocess development CML Carvalho, MR Aires‐Barros, JMS Cabral Biotechnology and bioengineering 66 (1), 17-34, 1999 | 292 | 1999 |
Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies PAJ Rosa, AM Azevedo, MR Aires-Barros Journal of Chromatography A 1141 (1), 50-60, 2007 | 270 | 2007 |
Partitioning of human antibodies in polyethylene glycol–sodium citrate aqueous two-phase systems AM Azevedo, AG Gomes, PAJ Rosa, IF Ferreira, AMMO Pisco, ... Separation and Purification Technology 65 (1), 14-21, 2009 | 261 | 2009 |
Partitioning in aqueous two-phase systems: fundamentals, applications and trends AL Grilo, M Raquel Aires-Barros, AM Azevedo Separation & Purification Reviews 45 (1), 68-80, 2016 | 260 | 2016 |
Magnetic separations in biotechnology L Borlido, AM Azevedo, ACA Roque, MR Aires-Barros Biotechnology advances 31 (8), 1374-1385, 2013 | 254 | 2013 |
Liquid− liquid extraction of proteins with reversed micelles MJ Pires, MR Aires‐Barros, JMS Cabral Biotechnology Progress 12 (3), 290-301, 1996 | 215 | 1996 |
Optimisation of aqueous two-phase extraction of human antibodies AM Azevedo, PAJ Rosa, IF Ferreira, MR Aires-Barros Journal of biotechnology 132 (2), 209-217, 2007 | 192 | 2007 |
Aqueous two-phase extraction as a platform in the biomanufacturing industry: economical and environmental sustainability PAJ Rosa, AM Azevedo, S Sommerfeld, W Bäcker, MR Aires-Barros Biotechnology advances 29 (6), 559-567, 2011 | 190 | 2011 |
Selective separation and purification of two lipases from Chromobacterium viscosum using AOT reversed micelles MR Aires‐Barros, JMS Cabral Biotechnology and bioengineering 38 (11), 1302-1307, 1991 | 168 | 1991 |
Carotenoid and lipid production by the autotrophic microalga Chlorella protothecoides under nutritional, salinity, and luminosity stress conditions L Campenni’, BP Nobre, CA Santos, AC Oliveira, MR Aires-Barros, ... Applied microbiology and biotechnology 97, 1383-1393, 2013 | 164 | 2013 |
Partitioning in aqueous two‐phase systems: Analysis of strengths, weaknesses, opportunities and threats RRG Soares, AM Azevedo, JM Van Alstine, MR Aires‐Barros Biotechnology journal 10 (8), 1158-1169, 2015 | 163 | 2015 |
Nonviral gene delivery to mesenchymal stem cells using cationic liposomes for gene and cell therapy C Madeira, RD Mendes, SC Ribeiro, JS Boura, MR Aires-Barros, ... BioMed Research International 2010 (1), 735349, 2010 | 152 | 2010 |
Affinity partitioning of human antibodies in aqueous two-phase systems PAJ Rosa, AM Azevedo, IF Ferreira, J De Vries, R Korporaal, HJ Verhoef, ... Journal of Chromatography A 1162 (1), 103-113, 2007 | 152 | 2007 |
Reverse micelles and protein biotechnology EP Melo, MR Aires-Barros, JMS Cabral Elsevier 7, 87-129, 2001 | 145 | 2001 |
Cutinase structure, function and biocatalytic applications CML Carvalho, MR Aires-Barros, J Cabral Electronic Journal of Biotechnology 1 (3), 28-29, 1998 | 145 | 1998 |
Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate GA Gomes, AM Azevedo, MR Aires-Barros, DMF Prazeres Separation and Purification Technology 65 (1), 22-30, 2009 | 143 | 2009 |
Purification of lipases MA Taipa, MR Aires-Barros, JMS Cabral Journal of biotechnology 26 (2-3), 111-142, 1992 | 142 | 1992 |
Application of aqueous two-phase systems to antibody purification: a multi-stage approach PAJ Rosa, AM Azevedo, S Sommerfeld, M Mutter, MR Aires-Barros, ... Journal of biotechnology 139 (4), 306-313, 2009 | 132 | 2009 |