| Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics PY Colin, B Kintses, F Gielen, CM Miton, G Fischer, MF Mohamed, ... Nature communications 6 (1), 10008, 2015 | 420 | 2015 |
| Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS) F Gielen, R Hours, S Emond, M Fischlechner, U Schell, F Hollfelder Proceedings of the National Academy of Sciences 113 (47), E7383-E7389, 2016 | 277 | 2016 |
| A microdroplet dilutor for high-throughput screening X Niu, F Gielen, JB Edel, AJ Demello Nature chemistry 3 (6), 437-442, 2011 | 255 | 2011 |
| Exploring sequence space in search of functional enzymes using microfluidic droplets P Mair, F Gielen, F Hollfelder Current Opinion in Chemical Biology 37, 137-144, 2017 | 116 | 2017 |
| A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition F Gielen, L van Vliet, BT Koprowski, SRA Devenish, M Fischlechner, ... Analytical chemistry 85 (9), 4761-4769, 2013 | 110 | 2013 |
| Electro-coalescence of digitally controlled droplets X Niu, F Gielen, AJ demello, JB Edel Analytical chemistry 81 (17), 7321-7325, 2009 | 106 | 2009 |
| Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures V Anagnostidis, B Sherlock, J Metz, P Mair, F Hollfelder, F Gielen Lab on a Chip 20 (5), 889-900, 2020 | 90 | 2020 |
| Materials and methods for droplet microfluidic device fabrication KS Elvira, F Gielen, SSH Tsai, AM Nightingale Lab on a Chip 22 (5), 859-875, 2022 | 66 | 2022 |
| Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets F Gielen, T Buryska, LV Vliet, M Butz, J Damborsky, Z Prokop, F Hollfelder Analytical chemistry 87 (1), 624-632, 2015 | 53 | 2015 |
| Long‐term perfusion culture of monoclonal embryonic stem cells in 3D hydrogel beads for continuous optical analysis of differentiation H Kleine‐Brüggeney, LD Van Vliet, C Mulas, F Gielen, CC Agley, ... Small 15 (5), 1804576, 2019 | 39 | 2019 |
| Quantitative affinity determination by fluorescence anisotropy measurements of individual nanoliter droplets F Gielen, M Butz, EJ Rees, M Erdelyi, T Moschetti, M Hyvönen, JB Edel, ... Analytical chemistry 89 (2), 1092-1101, 2017 | 28 | 2017 |
| Multi‐Object detector yolov4‐tiny enables high‐throughput combinatorial and spatially‐resolved sorting of cells in microdroplets L Howell, V Anagnostidis, F Gielen Advanced Materials Technologies 7 (5), 2101053, 2022 | 27 | 2022 |
| A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth WY Chen, E Avezov, SC Schlachter, F Gielen, RF Laine, HP Harding, ... Biophysical journal 108 (5), 999-1002, 2015 | 25 | 2015 |
| Controlled oil/water partitioning of hydrophobic substrates extending the bioanalytical applications of droplet-based microfluidics T Buryska, M Vasina, F Gielen, P Vanacek, L van Vliet, J Jezek, Z Pilat, ... Analytical chemistry 91 (15), 10008-10015, 2019 | 22 | 2019 |
| High-resolution local imaging of temperature in dielectrophoretic platforms F Gielen, F Pereira, AJ Demello, JB Edel Analytical chemistry 82 (17), 7509-7514, 2010 | 22 | 2010 |
| Dielectric cell response in highly conductive buffers F Gielen, AJ deMello, JB Edel Analytical Chemistry 84 (4), 1849-1853, 2012 | 20 | 2012 |
| Phenotyping single-cell motility in microfluidic confinement SA Bentley, H Laeverenz-Schlogelhofer, V Anagnostidis, J Cammann, ... Elife 11, e76519, 2022 | 19 | 2022 |
| Ultrahigh-throughput screening of single-cell lysates for directed evolution and functional metagenomics F Gielen, PY Colin, P Mair, F Hollfelder Protein engineering: methods and protocols, 297-309, 2018 | 19 | 2018 |
| Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2 S Rogers, C Zhang, V Anagnostidis, C Liddle, ML Fishel, F Gielen, ... Proceedings of the National Academy of Sciences 120 (39), e2217612120, 2023 | 13 | 2023 |
| Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats LH Lindenburg, T Pantelejevs, F Gielen, P Zuazua-Villar, M Butz, E Rees, ... Proceedings of the National Academy of Sciences 118 (46), e2017708118, 2021 | 12 | 2021 |