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Ashty Karim
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Cell-free gene expression: an expanded repertoire of applications
AD Silverman, AS Karim, MC Jewett
Nature Reviews Genetics 21 (3), 151-170, 2020
5202020
Metabolic engineering of muconic acid production in Saccharomyces cerevisiae
KA Curran, JM Leavitt, AS Karim, HS Alper
Metabolic engineering 15, 55-66, 2013
3542013
Cell‐free metabolic engineering: biomanufacturing beyond the cell
QM Dudley, AS Karim, MC Jewett
Biotechnology journal 10 (1), 69-82, 2015
3502015
Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale
FE Liew, R Nogle, T Abdalla, BJ Rasor, C Canter, RO Jensen, L Wang, ...
Nature biotechnology 40 (3), 335-344, 2022
3232022
Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications
AS Karim, KA Curran, HS Alper
FEMS yeast research 13 (1), 107-116, 2013
2642013
Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications
KA Curran, AS Karim, A Gupta, HS Alper
Metabolic engineering 19, 88-97, 2013
2612013
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
AS Karim, MC Jewett
Metabolic engineering 36, 116-126, 2016
2462016
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
AS Karim, QM Dudley, A Juminaga, Y Yuan, SA Crowe, JT Heggestad, ...
Nature Chemical Biology 16, 912-919, 2020
2032020
Design of synthetic yeast promoters via tuning of nucleosome architecture
KA Curran, NC Crook, AS Karim, A Gupta, AM Wagman, HS Alper
Nature communications 5 (1), 4002, 2014
1652014
A pressure test to make 10 molecules in 90 days: external evaluation of methods to engineer biology
A Casini, FY Chang, R Eluere, AM King, EM Young, QM Dudley, A Karim, ...
Journal of the American Chemical Society 140 (12), 4302-4316, 2018
1582018
In vitro prototyping of limonene biosynthesis using cell-free protein synthesis
QM Dudley, AS Karim, CJ Nash, MC Jewett
Metabolic Engineering 61, 251-260, 2020
1042020
Controlling cell-free metabolism through physiochemical perturbations
AS Karim, JT Heggestad, SA Crowe, MC Jewett
Metabolic engineering 45, 86-94, 2018
802018
Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin
L Zhuang, S Huang, WQ Liu, AS Karim, MC Jewett, J Li
Metabolic Engineering 60, 37-44, 2020
742020
Toward sustainable, cell-free biomanufacturing
BJ Rasor, B Vögeli, GM Landwehr, JW Bogart, AS Karim, MC Jewett
Current Opinion in Biotechnology 69, 136-144, 2021
692021
Cell-free synthetic biology for pathway prototyping
AS Karim, MC Jewett
Methods in enzymology 608, 31-57, 2018
652018
Cell-free styrene biosynthesis at high titers
WS Grubbe, BJ Rasor, A Krüger, MC Jewett, AS Karim
Metabolic Engineering 61, 89-95, 2020
602020
Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria
B Vögeli, L Schulz, S Garg, K Tarasava, JM Clomburg, SH Lee, A Gonnot, ...
Nature communications 13 (1), 3058, 2022
502022
A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of conjugate vaccines
KF Warfel, A Williams, DA Wong, SE Sobol, P Desai, J Li, YF Chang, ...
ACS synthetic biology 12 (1), 95-107, 2022
492022
High-throughput optimization cycle of a cell-free ribosome assembly and protein synthesis system
F Caschera, AS Karim, G Gazzola, AE d’Aquino, NH Packard, MC Jewett
ACS synthetic biology 7 (12), 2841-2853, 2018
452018
Cell‐free exploration of the natural product chemical space
JW Bogart, MD Cabezas, B Vögeli, DA Wong, AS Karim, MC Jewett
ChemBioChem 22 (1), 84-91, 2021
422021
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Articles 1–20